plenti cmv to neo dest Search Results


other lentiviral vectors  (Addgene inc)
93
Addgene inc other lentiviral vectors
a, Growth curve of SKD-HMEC left untreated or treated with OSM [10 ng/mL] for 1, 2, and 4 days. The mean cell number ± SEM (error bars) of all biological triplicates are presented for each time point. b-d, SKD-HMEC were serum-starved for 24 hours and then left untreated or subjected to a time course treatment with OSM [10 ng/mL] for .5, 2, 8, or 24 hours. At each time point, mRNA and protein were harvested from cells and subjected to (b) qRT-PCR analysis using primers targeting SOCS3 and SNAI1; (c) western analysis examining protein levels of P-STAT3, total STAT3, Snail, c-MYC, and Actin as a loading control; or (d) qRT-PCR analysis using primers targeting MYC or GLB1 to assess proliferation and senescence-associated gene changes as well as ZEB1 and SOD2 to assess EMT-associated gene changes. Error bars indicate mean ± SEM for three technical replicates. e, Flow cytometry of CD24 and CD44 surface-profile expression in GFP, OSM, H-Ras V12 (Ras-V12), Snail, Twist, and Zeb1-expressing SKD-HMEC 10 days post <t>lentiviral-infection.</t> Black numbers represent the percent of total cell population that are CD44LO (left) or CD44HI (right). f, Western analysis to confirm elevated protein levels in each respective SKD-HMEC derivative 10 days after infection with lentiviruses encoding GFP, OSM, Ras-V12, Snail, Twist, or Zeb1. g, Relative growth assays of SKD-HMEC derivatives expressing GFP, OSM, Ras-V12, Snail, Twist, or Zeb1 10 days post lentiviral-infection. Error bars indicate mean ± SEM for three technical replicates. h, Brightfield microscopy (10X) of senescence-associated β-galactosidase (SA-β-Gal) activity staining (dark) in GFP, Ras-V12, Snail, Twist, and Zeb1-expressing SKD-HMEC. i, qRT-PCR analysis of SNAI1 expression in SKD-HMEC 10 days after infection with lentiviruses encoding either GFP, Ras-V12, Twist, or Zeb1. Error bars indicate mean ± SEM of three independent experiments. Statistically significant differences are indicated with * when P < 0.05, ** when P < 0.01, *** when P < 0.001, and **** when P < 0.0001. Differences with a P value > 0.05 were considered non-significant and indicated with ns.
Other Lentiviral Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plenti+cmv+to+neo+dest/pmc08867383-245-0-6?v=Addgene+inc
Average 93 stars, based on 1 article reviews
other lentiviral vectors - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
pcmv spike  (Addgene inc)
93
Addgene inc pcmv spike
a, Growth curve of SKD-HMEC left untreated or treated with OSM [10 ng/mL] for 1, 2, and 4 days. The mean cell number ± SEM (error bars) of all biological triplicates are presented for each time point. b-d, SKD-HMEC were serum-starved for 24 hours and then left untreated or subjected to a time course treatment with OSM [10 ng/mL] for .5, 2, 8, or 24 hours. At each time point, mRNA and protein were harvested from cells and subjected to (b) qRT-PCR analysis using primers targeting SOCS3 and SNAI1; (c) western analysis examining protein levels of P-STAT3, total STAT3, Snail, c-MYC, and Actin as a loading control; or (d) qRT-PCR analysis using primers targeting MYC or GLB1 to assess proliferation and senescence-associated gene changes as well as ZEB1 and SOD2 to assess EMT-associated gene changes. Error bars indicate mean ± SEM for three technical replicates. e, Flow cytometry of CD24 and CD44 surface-profile expression in GFP, OSM, H-Ras V12 (Ras-V12), Snail, Twist, and Zeb1-expressing SKD-HMEC 10 days post <t>lentiviral-infection.</t> Black numbers represent the percent of total cell population that are CD44LO (left) or CD44HI (right). f, Western analysis to confirm elevated protein levels in each respective SKD-HMEC derivative 10 days after infection with lentiviruses encoding GFP, OSM, Ras-V12, Snail, Twist, or Zeb1. g, Relative growth assays of SKD-HMEC derivatives expressing GFP, OSM, Ras-V12, Snail, Twist, or Zeb1 10 days post lentiviral-infection. Error bars indicate mean ± SEM for three technical replicates. h, Brightfield microscopy (10X) of senescence-associated β-galactosidase (SA-β-Gal) activity staining (dark) in GFP, Ras-V12, Snail, Twist, and Zeb1-expressing SKD-HMEC. i, qRT-PCR analysis of SNAI1 expression in SKD-HMEC 10 days after infection with lentiviruses encoding either GFP, Ras-V12, Twist, or Zeb1. Error bars indicate mean ± SEM of three independent experiments. Statistically significant differences are indicated with * when P < 0.05, ** when P < 0.01, *** when P < 0.001, and **** when P < 0.0001. Differences with a P value > 0.05 were considered non-significant and indicated with ns.
Pcmv Spike, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plenti+cmv+to+neo+dest/pm41430043-261-11-12?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pcmv spike - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier

Image Search Results


a, Growth curve of SKD-HMEC left untreated or treated with OSM [10 ng/mL] for 1, 2, and 4 days. The mean cell number ± SEM (error bars) of all biological triplicates are presented for each time point. b-d, SKD-HMEC were serum-starved for 24 hours and then left untreated or subjected to a time course treatment with OSM [10 ng/mL] for .5, 2, 8, or 24 hours. At each time point, mRNA and protein were harvested from cells and subjected to (b) qRT-PCR analysis using primers targeting SOCS3 and SNAI1; (c) western analysis examining protein levels of P-STAT3, total STAT3, Snail, c-MYC, and Actin as a loading control; or (d) qRT-PCR analysis using primers targeting MYC or GLB1 to assess proliferation and senescence-associated gene changes as well as ZEB1 and SOD2 to assess EMT-associated gene changes. Error bars indicate mean ± SEM for three technical replicates. e, Flow cytometry of CD24 and CD44 surface-profile expression in GFP, OSM, H-Ras V12 (Ras-V12), Snail, Twist, and Zeb1-expressing SKD-HMEC 10 days post lentiviral-infection. Black numbers represent the percent of total cell population that are CD44LO (left) or CD44HI (right). f, Western analysis to confirm elevated protein levels in each respective SKD-HMEC derivative 10 days after infection with lentiviruses encoding GFP, OSM, Ras-V12, Snail, Twist, or Zeb1. g, Relative growth assays of SKD-HMEC derivatives expressing GFP, OSM, Ras-V12, Snail, Twist, or Zeb1 10 days post lentiviral-infection. Error bars indicate mean ± SEM for three technical replicates. h, Brightfield microscopy (10X) of senescence-associated β-galactosidase (SA-β-Gal) activity staining (dark) in GFP, Ras-V12, Snail, Twist, and Zeb1-expressing SKD-HMEC. i, qRT-PCR analysis of SNAI1 expression in SKD-HMEC 10 days after infection with lentiviruses encoding either GFP, Ras-V12, Twist, or Zeb1. Error bars indicate mean ± SEM of three independent experiments. Statistically significant differences are indicated with * when P < 0.05, ** when P < 0.01, *** when P < 0.001, and **** when P < 0.0001. Differences with a P value > 0.05 were considered non-significant and indicated with ns.

Journal: Molecular cancer research : MCR

Article Title: Aberrant induction of a mesenchymal/stem-cell program engages senescence in normal mammary epithelial cells.

doi: 10.1158/1541-7786.MCR-19-1181

Figure Lengend Snippet: a, Growth curve of SKD-HMEC left untreated or treated with OSM [10 ng/mL] for 1, 2, and 4 days. The mean cell number ± SEM (error bars) of all biological triplicates are presented for each time point. b-d, SKD-HMEC were serum-starved for 24 hours and then left untreated or subjected to a time course treatment with OSM [10 ng/mL] for .5, 2, 8, or 24 hours. At each time point, mRNA and protein were harvested from cells and subjected to (b) qRT-PCR analysis using primers targeting SOCS3 and SNAI1; (c) western analysis examining protein levels of P-STAT3, total STAT3, Snail, c-MYC, and Actin as a loading control; or (d) qRT-PCR analysis using primers targeting MYC or GLB1 to assess proliferation and senescence-associated gene changes as well as ZEB1 and SOD2 to assess EMT-associated gene changes. Error bars indicate mean ± SEM for three technical replicates. e, Flow cytometry of CD24 and CD44 surface-profile expression in GFP, OSM, H-Ras V12 (Ras-V12), Snail, Twist, and Zeb1-expressing SKD-HMEC 10 days post lentiviral-infection. Black numbers represent the percent of total cell population that are CD44LO (left) or CD44HI (right). f, Western analysis to confirm elevated protein levels in each respective SKD-HMEC derivative 10 days after infection with lentiviruses encoding GFP, OSM, Ras-V12, Snail, Twist, or Zeb1. g, Relative growth assays of SKD-HMEC derivatives expressing GFP, OSM, Ras-V12, Snail, Twist, or Zeb1 10 days post lentiviral-infection. Error bars indicate mean ± SEM for three technical replicates. h, Brightfield microscopy (10X) of senescence-associated β-galactosidase (SA-β-Gal) activity staining (dark) in GFP, Ras-V12, Snail, Twist, and Zeb1-expressing SKD-HMEC. i, qRT-PCR analysis of SNAI1 expression in SKD-HMEC 10 days after infection with lentiviruses encoding either GFP, Ras-V12, Twist, or Zeb1. Error bars indicate mean ± SEM of three independent experiments. Statistically significant differences are indicated with * when P < 0.05, ** when P < 0.01, *** when P < 0.001, and **** when P < 0.0001. Differences with a P value > 0.05 were considered non-significant and indicated with ns.

Article Snippet: Other lentiviral vectors also obtained from Addgene were gifts from Eric Campeau and Paul Kaufman, including pLenti CMV-Neo GFP (657–2) (plasmid # 17447), pLenti CMV-Puro GFP (658–5) (plasmid # 17448), pENTR4 no ccDB (686–1) (plasmid # 17424), pLenti CMV-Puro DEST (w118–1) (plasmid # 17452), pLenti CMV-Neo DEST (705–1) (plasmid # 17392), and pLenti CMV/TO Neo DEST (685–3) (plasmid # 17292).

Techniques: Quantitative RT-PCR, Western Blot, Flow Cytometry, Expressing, Infection, Microscopy, Activity Assay, Staining

Fold-change expression of senescence-, EMT-, and CSC-associated genes in OSM-induced CD44 LO and CD44 HI populations. SKD-HMEC were infected with lentiviruses encoding either GFP or OSM. 10 days after lentiviral infection, cells were subjected to FACS to purify three populations: CD44 LO GFP-control cells (GFP-44 LO ) and two sub-populations of OSM-expressing cells representing the lowest CD44 expressing cells (OSM-44 LO ) or the highest level of CD44 (OSM-44 HI ). After FACS-purification, mRNA was harvested from each sorted population and subjected to qRT-PCR analysis to compare the expression of senescence-associated, EMT-associated, and CSC-associated genes. Data shown for relative gene expression represent the mean of technical triplicates for one experiment normalized to GFP-44 LO ; Statistical significance was determined by performing a two-tailed unpaired Student’s t -test using the Holm-Sidak method without assuming a consistent SD. A value of P < 0.05 was considered statistically significant.

Journal: Molecular cancer research : MCR

Article Title: Aberrant induction of a mesenchymal/stem-cell program engages senescence in normal mammary epithelial cells.

doi: 10.1158/1541-7786.MCR-19-1181

Figure Lengend Snippet: Fold-change expression of senescence-, EMT-, and CSC-associated genes in OSM-induced CD44 LO and CD44 HI populations. SKD-HMEC were infected with lentiviruses encoding either GFP or OSM. 10 days after lentiviral infection, cells were subjected to FACS to purify three populations: CD44 LO GFP-control cells (GFP-44 LO ) and two sub-populations of OSM-expressing cells representing the lowest CD44 expressing cells (OSM-44 LO ) or the highest level of CD44 (OSM-44 HI ). After FACS-purification, mRNA was harvested from each sorted population and subjected to qRT-PCR analysis to compare the expression of senescence-associated, EMT-associated, and CSC-associated genes. Data shown for relative gene expression represent the mean of technical triplicates for one experiment normalized to GFP-44 LO ; Statistical significance was determined by performing a two-tailed unpaired Student’s t -test using the Holm-Sidak method without assuming a consistent SD. A value of P < 0.05 was considered statistically significant.

Article Snippet: Other lentiviral vectors also obtained from Addgene were gifts from Eric Campeau and Paul Kaufman, including pLenti CMV-Neo GFP (657–2) (plasmid # 17447), pLenti CMV-Puro GFP (658–5) (plasmid # 17448), pENTR4 no ccDB (686–1) (plasmid # 17424), pLenti CMV-Puro DEST (w118–1) (plasmid # 17452), pLenti CMV-Neo DEST (705–1) (plasmid # 17392), and pLenti CMV/TO Neo DEST (685–3) (plasmid # 17292).

Techniques: Expressing, Infection